ficoll paque medium Search Results


ficoll paque plus medium cushion  (Cytiva Europe)
98
Cytiva Europe ficoll paque plus medium cushion
Ficoll Paque Plus Medium Cushion, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
ficoll paque plus medium cushion - by Bioz Stars, 2026-08
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separation medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc separation medium
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Separation Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pmc07481096-88-18-29?v=STEMCELL+Technologies+Inc
Average 90 stars, based on 1 article reviews
separation medium - by Bioz Stars, 2026-08
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ficoll paque plus medium  (Ficoll-Paque Pharmacia)
86
Ficoll-Paque Pharmacia ficoll paque plus medium
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Ficoll Paque Plus Medium, supplied by Ficoll-Paque Pharmacia, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pm38455046-55-26-26?v=Ficoll-Paque+Pharmacia
Average 86 stars, based on 1 article reviews
ficoll paque plus medium - by Bioz Stars, 2026-08
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ficoll paque density gradient medium  (Cytiva Europe)
97
Cytiva Europe ficoll paque density gradient medium
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Ficoll Paque Density Gradient Medium, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/bio_rxiv__64898__2026__03__18__712552-122-15-19?v=Cytiva+Europe
Average 97 stars, based on 1 article reviews
ficoll paque density gradient medium - by Bioz Stars, 2026-08
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ficall paque premium mononuclear cell separation medium  (Cytiva Europe)
95
Cytiva Europe ficall paque premium mononuclear cell separation medium
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Ficall Paque Premium Mononuclear Cell Separation Medium, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pm40394087-207-12-19?v=Cytiva+Europe
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ficall paque premium mononuclear cell separation medium - by Bioz Stars, 2026-08
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ficoll paque premium  (Cytiva Europe)
96
Cytiva Europe ficoll paque premium
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Ficoll Paque Premium, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pmc05395139-69-4-9?v=Cytiva+Europe
Average 96 stars, based on 1 article reviews
ficoll paque premium - by Bioz Stars, 2026-08
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endocult liquid medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc endocult liquid medium
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Endocult Liquid Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pmc03882533-105-15-18?v=STEMCELL+Technologies+Inc
Average 90 stars, based on 1 article reviews
endocult liquid medium - by Bioz Stars, 2026-08
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lymphoprep™  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc lymphoprep™
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Lymphoprep™, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pmc05528969-829-13-11?v=STEMCELL+Technologies+Inc
Average 90 stars, based on 1 article reviews
lymphoprep™ - by Bioz Stars, 2026-08
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ficoll-paque plus density medium separation  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc ficoll-paque plus density medium separation
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Ficoll Paque Plus Density Medium Separation, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pmc03422413-75-10-16?v=STEMCELL+Technologies+Inc
Average 90 stars, based on 1 article reviews
ficoll-paque plus density medium separation - by Bioz Stars, 2026-08
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stemspan sfem medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc stemspan sfem medium
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Stemspan Sfem Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pm36035798-42-74-79?v=STEMCELL+Technologies+Inc
Average 90 stars, based on 1 article reviews
stemspan sfem medium - by Bioz Stars, 2026-08
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ficoll paque medium  (Beijing Solarbio Science)
99
Beijing Solarbio Science ficoll paque medium
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Ficoll Paque Medium, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pmc11622586-254-14-16?v=Beijing+Solarbio+Science
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ficoll paque medium - by Bioz Stars, 2026-08
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density gradient medium ficoll  (Cytiva Europe)
96
Cytiva Europe density gradient medium ficoll
Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte <t>separation</t> medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.
Density Gradient Medium Ficoll, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ficoll+paque+medium/pmc08466236-64-6-12?v=Cytiva+Europe
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Image Search Results


Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte separation medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.

Journal: Journal for Immunotherapy of Cancer

Article Title: Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation

doi: 10.1136/jitc-2020-001223

Figure Lengend Snippet: Identification of further technical variables in MDSC immunomonitoring. Participating centers stained for MDSC subsets in healthy donor controls. Beforehand every participant received the identical batch of blood collection tubes. From the same donor sodium citrate-, EDTA and heparin blood was collected and PBMC were stained for MDSC subsets in PBMC. Lymphocyte separation medium was free of choice. MDSC frequency was determined in the core lab as described for . Influence of anticoagulants (A) and lymphocyte separation medium (B) on total frequency of putative ‘MDSC subsets’ in healthy individuals with mean and SD is shown. Kruskal-Wallis was used for statistical analysis. Results were considered significant at *p≤0.05, **p≤0.001 and ***p≤0.0001. M-MDSC, monocytic myeloid-derived suppressor cell; PBMC, peripheral blood mononuclear cell; PMN, polymorphonuclear.

Article Snippet: Before separation blood was admixed with the same volume of phosphate-buffered saline (PBS) and overlayed on 1.077 g/mL separation medium (Biocoll, Merck Millipore; Ficoll-Paque, GE healthcare; Pancoll, PAN-Biotech; Lymphoprep, Stemcell Technologies).

Techniques: Staining, Derivative Assay

Standardization of anticoagulant and separation medium reduces intercenter variability of PMN-MDSC frequencies. All participants used sodium citrate as anticoagulant and Biocoll as separation medium and determined the frequency of PMN-MDSC in healthy donor controls. Data were compared with data obtained for . (A) Frequency of PMN-MDSC in Step1 ( , free choice of anticoagulant and separation medium) and step 2 (sodium citrate and Biocoll) from all healthy blood donors in the five centers is shown (plus mean and SD). (B) The mean frequency was determined for each of the five participating centers and (C) % cv was calculated. In all panels the comparison to step 1 (data from ) is shown. F test was used for statistical analysis. Results were considered significant at *p≤0.05 and ****p≤0.0001. PBMC; peripheral blood mononuclear cell.

Journal: Journal for Immunotherapy of Cancer

Article Title: Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation

doi: 10.1136/jitc-2020-001223

Figure Lengend Snippet: Standardization of anticoagulant and separation medium reduces intercenter variability of PMN-MDSC frequencies. All participants used sodium citrate as anticoagulant and Biocoll as separation medium and determined the frequency of PMN-MDSC in healthy donor controls. Data were compared with data obtained for . (A) Frequency of PMN-MDSC in Step1 ( , free choice of anticoagulant and separation medium) and step 2 (sodium citrate and Biocoll) from all healthy blood donors in the five centers is shown (plus mean and SD). (B) The mean frequency was determined for each of the five participating centers and (C) % cv was calculated. In all panels the comparison to step 1 (data from ) is shown. F test was used for statistical analysis. Results were considered significant at *p≤0.05 and ****p≤0.0001. PBMC; peripheral blood mononuclear cell.

Article Snippet: Before separation blood was admixed with the same volume of phosphate-buffered saline (PBS) and overlayed on 1.077 g/mL separation medium (Biocoll, Merck Millipore; Ficoll-Paque, GE healthcare; Pancoll, PAN-Biotech; Lymphoprep, Stemcell Technologies).

Techniques: Comparison

Expression of molecules associated with T cell suppression expression of PD-L1 and LOX-1 on MDSC subsets in patients and healthy donor controls was determined using the harmonized flow cytometry labeling protocol, Biocoll as standard separation medium and sodium citrate as standard anticoagulant. Flow cytometry analysis was performed in the core lab to ensure standardized gating. Staining intensity of PD-L1 (A) and LOX-1 (B) on MDSC-subsets was determined in five different disease settings. Delta median (median signal intensity of antibody minus median signal intensity of isotype control) is shown. Data are depicted as mean and SD is shown. CVD, cardiovascular disease; M-MDSC, monocytic myeloid-derived suppressor cells; PD-L1, programmed death-ligand 1; PMN, polymorphonuclear.

Journal: Journal for Immunotherapy of Cancer

Article Title: Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation

doi: 10.1136/jitc-2020-001223

Figure Lengend Snippet: Expression of molecules associated with T cell suppression expression of PD-L1 and LOX-1 on MDSC subsets in patients and healthy donor controls was determined using the harmonized flow cytometry labeling protocol, Biocoll as standard separation medium and sodium citrate as standard anticoagulant. Flow cytometry analysis was performed in the core lab to ensure standardized gating. Staining intensity of PD-L1 (A) and LOX-1 (B) on MDSC-subsets was determined in five different disease settings. Delta median (median signal intensity of antibody minus median signal intensity of isotype control) is shown. Data are depicted as mean and SD is shown. CVD, cardiovascular disease; M-MDSC, monocytic myeloid-derived suppressor cells; PD-L1, programmed death-ligand 1; PMN, polymorphonuclear.

Article Snippet: Before separation blood was admixed with the same volume of phosphate-buffered saline (PBS) and overlayed on 1.077 g/mL separation medium (Biocoll, Merck Millipore; Ficoll-Paque, GE healthcare; Pancoll, PAN-Biotech; Lymphoprep, Stemcell Technologies).

Techniques: Expressing, Flow Cytometry, Labeling, Staining, Control, Derivative Assay